Single-cell methylation whole genome sequencing: cell atlases in pig to human xenotransplantation

Only 1 in 4 people on US transplant waitlists will received an organ. Transplanting pig xenografts into human decedents affords a unique opportunity to deeply profile and analyze longitudinal blood and tissue timepoints. Recent advances in single-cell and omic technologies have afforded unparalleled abilities to deeply profile blood and tissue in an unbiased (untargeted) fashion. Here we describe the generation of large scale single-cell methylation whole genome sequencing cell atlases in pig to human xenotransplants linked to other omics in attempt to generate diagnostic and prognostic markers of xenograft damage.

 

3 Learning Objectives