mouse monoclonal IgG2a; PDK3 Antibody (A-4) is an IgG2a κ mouse monoclonal PDK3 antibody (also designated PDHK3 antibody) that detects the PDK3 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. PDK3 Antibody (A-4) is available as both the non-conjugated anti-PDK3 antibody form, as well as multiple conjugated forms of anti-PDK3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Pyruvate dehydrogenase kinase family members (PDK1, 2, 3, 4) are serine kinases that catalyze phosphorylation of the E1α subunit of the pyruvate dehydrogenase complex (PDC). PDC activity is controlled through phosphorylation and dephosphorylation of the E1α subunit, which leads to inactivation and reactivation, respectively. PDK3 binding to a free lipoyl domain (L2) in dihydrolypoyl acetyltransferase (E2), which comprises the core of PDC, leads to a large increase in E1α phosphorylation. Upregulation of PDK isoenzymes occurs during starvation conditions, where acetyl-CoA is alternatively generated through fatty acid oxidation. PDKs contain five conserved regions and are mechanistically similar to bacterial His-kinases in that both require histidine residues for activity. In mammals, transcripts for PDK3 are most abundant in testis and moderately expressed in heart and skeletal muscle.