mouse monoclonal IgG2b; paxillin Antibody (B-2) is an IgG2b κ mouse monoclonal paxillin antibody (also designated PXN antibody) that detects the paxillin protein of mouse, rat and human origin by WB, IP, IF and ELISA. paxillin Antibody (B-2) is available as both the non-conjugated anti-paxillin antibody form, as well as multiple conjugated forms of anti-paxillin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Paxillin is a focal adhesion phosphoprotein that is localized to the cytoskeleton. Phosphorylation of paxillin has been shown to occur in response to PDGF treatment, v-src transformation or cross-linking of integrins. FAK (focal adhesion kinase) and PYK2 have been shown to phosphorylate paxillin. FAK phosphorylates paxillin specifically on Tyr 118 in vitro. However, FAK phosphorylation does not seem to be required for the recruitment of paxillin to cell adhesion sites. Paxillin may play a role in signal transduction, regulation of cell morphology and the recruitment of structural and signaling molecules to focal adhesions. It has been shown that the amount of paxillin is reduced in mitotic cells by proteolytic downregulation and that paxillin is alternatively phosphorylated on serine rather than on tyrosine and serine during mitosis.