Leica VT1200 | Leica Microsystems

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Semi-Automatic Vibrating Blade Microtome

The semi-automatic Leica VT1200 vibrating blade microtome is designed for sectioning fresh specimens in neuropathology (fresh brain slicing), neurophysiology (patch-clamping), and electrophysiology.

The instrument is recommended for users who prefer to control sectioning parameters such as section thickness and cutting stroke manually for each individual section. To achieve sections that retain viable cells on the section surface, the vertical deflection of the blade can be measured with the Leica Vibrocheck measurement device and minimized with the innovative blade holder.

The instrument was designed in collaboration with Prof. Dr. Peter Jonas (previously at the Physiology department of the University of Freiburg, Germany, now at Institute of Science and Technology, Klosterneuburg, Austria) and his former group.

FOR RESEARCH USE ONLY. Not For Use In Diagnostic Procedures.

Citations (1)

(1) Improved reproducibility in preparing precision-cut liver tissue slices.

Cytotechnology

March 12, 2025

Zimmermann M, Lampe J, Lange S, Smirnow I, Königsrainer A, Hann-von-Weyhern C, Fend F, Gregor M, Bitzer M, Lauer UM

Precision-cut liver tissue slices (PCLS) have been used for decades to study pharmacological metabolism as well as toxicology and efficacy of novel substances on primary material under standardized conditions. Slicing of primary liver tissue has been done using different slicing machines. Since there has been great variability in the results, we sought to compare the reproducibility of tissue slices generated using the newly developed Leica VT1200 S vibrating blade microtome with Vibrocheck (LV) and the Krumdieck tissue slicer (KD) which has been the standard apparatus for this application so far. Liver samples from five different species (human, pig, cattle, rat, mouse) were cut and the reproducibility of slice thickness was analyzed by cross sectioning the PCLS. The quality of the sliced tissue was determined via measurement of the ATP content. As a result, we found an improved accuracy and reproducibility of rat, mouse and human tissue slices using the new Leica vibrating blade microtome.